NUCLEIC ACID ELECTROPHORESIS. - With 62 figures and 10 tables

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Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA). Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described in detail. The required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers addresses, helpful Internet resources as well as specific applications, e.g. in legal medicine, microbiology and agriculture, are presented by leading experts.

TÉLÉCHARGER
AUTEUR Dietmar Tietz
DATE DE PUBLICATION 1998-Oct-27
TAILLE DU FICHIER 10,92 MB
LIRE EN LIGNE

Highly specific enrichment of rare nucleic acid fractions ...

Agarose gel electrophoresis is a routinely used method for separating proteins, DNA or RNA. (Kryndushkin et al., 2003). Nucleic acid molecules are size separated by the aid of an electric field ...

Nucleic Acid Gel Electrophoresis—A Brief Overview and ...

Capillary electrophoresis separations are significant because they provide fast separations of limited sample volumes. Following reports of outstanding separation efficiencies of amines, amino acids, and peptides achieved using a 75-μm-inner-diameter glass capillary [1, 2], the technology was quickly adapted to DNA [3, 4].

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