NUCLEIC ACID ELECTROPHORESIS. - With 62 figures and 10 tables

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Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA). Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described in detail. The required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers addresses, helpful Internet resources as well as specific applications, e.g. in legal medicine, microbiology and agriculture, are presented by leading experts.

TÉLÉCHARGER
AUTEUR Dietmar Tietz
DATE DE PUBLICATION 1998-Oct-27
TAILLE DU FICHIER 1,14 MB
LIRE EN LIGNE

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Nucleic Acid Electrophoresis The Standard in Quality, Precision and Performance. 2 BioResearch Nucleic Acid Electrophoresis Content Introduction 3 FlashGel™ System 4-6 Reliant™ Minigels 7-8 Latitude™ HT Gels and Midigels 9-10 Agarose 11-13 Ladders and Markers for DNA and RNA 14-15 Stains 16 AccuGENE™ Buffers 17 GelBond® Film 19. 3 Lonza is a leading manufacturer and trusted ...

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Electrophoresis is a method of separation and purification of macromolecules such as nucleic acids (DNA and RNA) and proteins based on the net charge, size and conformation on a matrix. Nucleic acids have an overall negative charge due to the presence of phosphate backbone. Therefore they move towards the anode at the migration rate that depends solely on their size. Proteins contain an ...

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